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Phospho-H2AX (Ser139) Rabbit Polyclonal Antibody (FITC)
Catalog Number: orb9001
| Catalog Number | orb9001 |
|---|---|
| Category | Antibodies |
| Description | Phospho-H2AX (Ser139) Rabbit Polyclonal Antibody (FITC) |
| Target | H2AX |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | FITC |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Porcine, Rabbit |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Histone H2AX around the phosphorylation site of Tyr139 QA(p-S)QE |
| RRID | AB_10918007 |
| Tested applications | FC, IF |
| Dilution range | Flow-Cyt=2ug/Test, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Alternative names | Phospho-Histone H2A.X (Ser140); H2AX_HUMAN; 2A.X v Read more... |
| Note | For research use only |
Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-H2AFX antibody (orb9001), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
