Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb8204
• Category: Antibodies
• Description: Phospho-GSK-3 Beta (Ser21+Ser29) Rabbit Polyclonal Antibody (FITC)
• Target: GSK3B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Sheep
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser21+Ser29 QP(p-S)AFGSMKV(p-S)RD
• RRID: AB_10928761
• Tested applications: ICC, IF
• Dilution range: IF=1:100-500, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: GSK3B(phospho-Ser21+Ser29); GSK3B(phospho-S21+S29)
• Research Area: Cancer Research, Cardiovascular Research, Cell Bio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-GSK-3 Beta (Ser21+Ser29) antibody (orb8204), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.