Phospho-FSCN1 (Ser39) Rabbit Polyclonal Antibody

• Catalog Number: orb11266
• Category: Antibodies
• Description: Phospho-FSCN1 (Ser39) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39 AS(p-S)LK
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: FSCN1
• UniProt ID: Q16658
• RRID: AB_10746450
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Fascin(phospho Ser39); Fascin(phospho S39); 55 kDa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-FSCN1 (Ser39)) polyclonal Antibody, Unconjugated (orb11266) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control: U937 (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 3 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FSCN1 (Ser39)) Polyclonal Antibody, Unconjugated (orb11266) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse Spleen tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Rat Testis tissue lysates, Lane 6: Rat Spleen tissue lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human K562 cell lysates, Lane 9: Human SH-SY5Y cell lysates, Lane 10: Human U-2os cell lysates, Primary: Anti-phospho-FSCN1 (Ser39) (orb11266) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 51 kDa.

Sample: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-FSCN1 (Ser39) (orb11266) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Tissue/Cell: musle of mouse embryo, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-FSCN1 (Ser39) Polyclonal Antibody, Unconjugated (orb11266) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.