Phospho-ASK1 (Thr845) Rabbit Polyclonal Antibody

• Catalog Number: orb6390
• Category: Antibodies
• Description: Phospho-ASK1 (Thr845) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ASK1 around the phosphorylation site of Thr845 TE(p-T)FT
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 155 kDa
• Target: MAP3K5
• UniProt ID: Q99683
• RRID: AB_10954285
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ASK1(phospho Thr845); Mitogen-activated protein ki
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jiaxuan He 1 2, Siyuan Li 1, Yunpeng Teng 1, Hongfei Xiong 1, Zhuang Wang 1, Xiaoyao Han 1, Wei Gong 2, Ya Gao Increasing expression of dual-specificity phosphatase 12 mitigates oxygen-glucose deprivation/reoxygenation-induced neuronal apoptosis and inflammation through inactivation of the ASK1-JNK/p38 MAPK pathway Autoimmunity, 57, 2345919 (2024)

PubMed: 38721693

Applications

WB

Reactivity

Mouse

Sample: Lane 8: Cerebrum (Rat) Lysate at 40 ug, Lane 9: Stomach (Rat) Lysate at 40 ug, Primary: Anti-phospho-ASK1 (Thr845) (orb6390) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 154 kD, Observed band size: 154 kD.

Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-phospho-ASK1 (Thr845) antibody (orb6390), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-phospho-RelB (Ser551) antibody (orb6390), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-phospho-ASK1 (Thr845) antibody (orb6390), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Brain (Rat) Lysate at 30 ug, Lane 2: Heart (Rat) Lysate at 30 ug, Primary: Anti-phospho-ASK1 (Thr845) (orb6390) at 1:200 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 155kD, Observed band size: 155kD.