Phospho-AKT1(Thr308)) Antibody

• Catalog Number: orb1931044
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This AKT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Thr308 of human AKT1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P31749
• MW: 55686 Da
• Tested applications: DOT, IF
• Dilution range: IF: 1:10~50, DB: 1:500
• Clone Number: RB27685
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RAC-alpha serine/threonine-protein kinase, Protein
• Note: For research use only
• NCBI: NP_001014431.1, NP_005154.2, NP_001014432.1

Dot blot analysis of anti-Phospho-AKT1 (Thr308) Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Confocal immunofluorescent analysis of Phospho-AKT1 (Thr308) Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).