Phospho-AKT (Ser473) Antibody

• Catalog Number: orb1294322
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AKT (Phospho-ser473)
• Target: Phospho-AKT (Ser473)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide corresponding to residues surrounding Ser473 of human AKT
• Tested applications: IF, WB
• Dilution range: Western Blot 1:1000 – 1:2000 Immunofluorescence 1:200 – 1:400
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

anti-Phospho-AKT (Ser473) antibody at 1/1000 dilution, Lysates/proteins at 20 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294322 overnight at 4°C.

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294322 overnight at 4°C. Anti-Phospho-Akt (Ser473) antibody - at 1/1000 dilution Lysates at 30 µg per lane.