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Catalog Number | orb93989 |
---|---|
Category | Tools |
Description | Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended. The enzyme is highly purified and free of bacterial DNA. |
Clonality | Recombinant |
Concentration | 2.5 units/µl |
Form/Appearance | liquid |
Storage | store at -20 °C |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |