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| Catalog Number | orb1733709 |
|---|---|
| Category | Tools |
| Description | Pfu-X Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Pfu DNA polymerase, but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction but does not possess a 5'→3' exonuclease replacement activity. Its inherent 3'→5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Pfu-X Polymerase-generated PCR fragments are blunt-ended. The enzyme is highly purified and free of bacterial DNA. |
| Form/Appearance | liquid |
| Concentration | 2.5 units/µl |
| Storage | store at -20 °C. avoid freeze/thaw cycles |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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