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P4HB Antibody
SKU: orb629775
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution range | WB: 1:500-1:1000; IP: 1:200-1:1000; IHC: 1:100-1:400; IF: 1:10-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | prolyl 4-hydroxylase, beta polypeptide |
| Target | P4HB |
| Molecular Weight | 57kd |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Disclaimer | For research use only |
Alternative Names
−DSI, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase
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COLO 320 cells were subjected to SDS PAGE followed by western blot with orb629775 (PDIA1 antibody) at dilution of 1:500.
Immunofluorescent analysis of HEK-293 cells using orb629775 (PDI antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
IP Result of anti-PDI (IP: orb629775, 3 ug; Detection: orb629775 1:300) with mouse liver tissue lysate 4000 ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using orb629775 (PDI Antibody) at dilution of 1:200.
Quick Database Links
Gene Symbol
P4HB
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
P4HB Antibody (orb629775)
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