Orexin receptor 1+2 Rabbit Polyclonal Antibody

SKU: orb11198

Description

Orexin receptor 1+2 Rabbit Polyclonal Antibody

Research Area

G Protein-Coupled Receptors, GPCR Signaling Pathway, Metabolism, Obesity

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Orexin receptor 1/2 (321-425/444aa)
• Target: Hcrtr1
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ORXR1; OX1R; OXR1; ox-1-R; ox1-R; Hctr1; OX1R_HUMAN; HCRTR1; Hypocretin receptor type 1; Orexin receptor type 1 (Ox-1-R | Ox1-R | Ox1R); OX1R_MOUSE; OX1R_RAT; ORXR2; OX2R; OXR2; OX2aR; OX2bR; mOX2aR; mOX2bR; mOXR2; ox-2-R; ox2-R; OX2R_HUMAN; HCRTR2; Hypocretin receptor type 2; OX2R_MOUSE; Mox2r; OX2R_RAT;

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (orb11198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse Cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Orexin receptor 1+2) Polyclonal Antibody, Unconjugated (orb11198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebral cortex (Mouse) Lysate at 40 ug, Hippocampus (Mouse) Lysate at 40 ug, Brain (Mouse) Lysate at 40 ug, Brain (Rat) Lysate at 40 ug, Primary: Anti-Orexin receptor 1+2 (orb11198) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Primary: Anti-Orexin receptor 1+2 (orb11198) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

Hcrtr1

UniProt

O43613

Sun, Haoyu et al. CD200R, a co-inhibitory receptor on immune cells, predicts the prognosis of human hepatocellular carcinoma Immunol Lett, 178, 105-113 (2016)

PubMed: 27562325