NGFR/p75NTR Rabbit Polyclonal Antibody

• Catalog Number: orb11125
• Category: Antibodies
• Description: NGFR/p75NTR Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NGFR (301-400/427aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: NGFR
• UniProt ID: P08138
• RRID: AB_10761319
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: TNR16_HUMAN; Tumor necrosis factor receptor superf
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Mouse spleen (blue). Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (orb11125), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: (mo) nephrocyte (blue), Isotype Control Antibody: Rabbit IgG-AF647 (orange), Primary Antibody Dilution: 5 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Blank control: Mouse brain. Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (orb11125), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.