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Catalog Number | orb749721 |
---|---|
Category | Antibodies |
Description | It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | Mouse IgG1, kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Immunogen | NGFR from A875 melanoma cells was used as the immunogen for the NGF Receptor antibody. Its epitope is within amino acids 1-160 of the extracellular domain of NGFR/NTR. |
UniProt ID | P08138 |
Tested applications | IHC-P |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Application notes | Optimal dilution of the p75NTR antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Antibody Type | Primary Antibody |
Clone Number | SPM299 |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Hazard Information | This NGF Receptor antibody is available for research use only. |
Note | For research use only |
IHC analysis of formalin-fixed, paraffin-embedded human adrenal gland stained with NGF Receptor antibody (clone SPM299).
IHC analysis of formalin-fixed, paraffin-embedded human melanoma stained with NGF Receptor antibody (clone SPM299).
SDS-PAGE analysis of purified, BSA-free NGF Receptor antibody (clone SPM299) as confirmation of integrity and purity.
IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |