Nestin Rabbit Polyclonal Antibody

SKU: orb1294409

Description

Rabbit polyclonal antibody to Nestin antibody (NES)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Target: Nestin / NES
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

Anti-Nestin antibody at 1/1000 dilution, Lysates at 30 µg per lane.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294409 anti-Nestin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with orb1294409 at 1/100.

Sample: Neuron 2A, Nestin antibody (orb1294409): 1-1000.

Quick Database Links

Gene Symbol

Nestin / NES