NCOA4 Antibody

SKU: orb1238076

Description

NCOA4 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine, Monkey, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-NCOA4 antibody (orb1238076) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.
• Target: NCOA4
• Molecular Weight: Predicted: 70-74 kDObserved: 70-74 kD
• Purification: NCOA4 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: NCOA4 orb1238076, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of NCOA4 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with orb1238076 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of NCOA4 in Human lung. Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of NCOA4 in Mouse lung. Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of NCOA4 in Rat lung. Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (orb1238076) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

NCOA4

UniProt

Q13772

RefSeq:Q13772