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NCOA4 Rabbit Polyclonal Antibody

Catalog Number: orb221641

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb221641
CategoryAntibodies
DescriptionNCOA4 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Mouse, Porcine, Rat, Sheep
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human NCOA4 (1-100/614aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW70 kDa
TargetNCOA4
UniProt IDQ13772
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative names70 kDa androgen receptor activator; 70 kDa androge
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NoteFor research use only
Expiration Date12 months from date of receipt.
NCOA4 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NCOA4) Polyclonal Antibody, Unconjugated (orb221641) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

NCOA4 Rabbit Polyclonal Antibody

Sample: Lane 1: HT1080 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: U937 (Human) Cell Lysate at 30 ug, Primary: Anti-NCOA4 (orb221641) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 85 kD.

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    IH,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 200 μl, 100 μl
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    IH,  WB

    Human, Mouse, Rat

    Rabbit

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    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

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    WB

    Bovine, Canine, Equine, Porcine, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    WB

    Bovine, Canine, Equine, Guinea pig, Human, Rabbit, Rat

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μl