NCF4 Antibody

SKU: orb1247225

Description

NCF4 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 38kDa band observed in lysates of cell line Daudi (calculated MW of 39.0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-KDFPEEDDPTN
• Target: NCF4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa , CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)

orb1247225 (0.5 ug/ml) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing P40PHOX and probed with orb1247225 (mock transfection in first lane).

orb1247225 (2.5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

NCF4

UniProt

Q15080

RefSeq (Protein):NP_038202.1, NP_000622.2