NASP Antibody

SKU: orb1256714

Description

NASP Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
• Target: NASP
• Molecular Weight: Observed: 85kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nuclear autoantigenic sperm protein, NASP, NASP

Western blot analysis of extracts of various cell lines, using NASP antibody (orb1256714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spleen using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat stomach using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendicitis using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using TOM20 antibody (orb1256783) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human adenomyosis using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using NASP Antibody (orb1256714) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

NASP

UniProt

P49321