N6-Propargyl-adenosine

SKU: orb532419

Description

N6-propargyl-adenosine (N6pA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. N6pA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I, II and III and posttranscription-ally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

• Application Notes:
  mRNA poly(A) tail synthesis monitoring. ; Spectroscopic Propertie: max 262 nm, 18.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 305.29 g/mol; Detected MW: 305.11 g/mol
• Purity: 95% (HPLC)
• Formula: C₁₃H₁₅N₅O₄
• SMILES: c1(c2c(ncn1)n(cn2)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)NCC#C

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only