MYLK Antibody

SKU: orb1256025

Description

MYLK Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1765-1914 of human MYLK (NP_444253.3).
• Target: MYLK
• Molecular Weight: Observed: 170kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

myosin, light polypeptide kinase, myosin light chain kinase, MSTP083, MLCK210, MLCK108, MLCK, KRP, FLJ12216, DKFZp686I10125, myosin, light chain kinase, MYLK, MLCK1, MYLK1

Western blot analysis of extracts of various cell lines, using MYLK antibody (orb1256025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human stomach using MYLK antibody (orb1256025) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using MYLK antibody (orb1256025) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using MYLK antibody (orb1256025) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

MYLK

UniProt

Q15746