Human Myosin Light Chain Kinase (MYLK) ELISA Kit

SKU: orb776156

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain Kinase(MYLK).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYLK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYLK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYLK in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.064 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

KRP ELISA Kit, MLCK108 ELISA Kit, MLCK210 ELISA Kit, MSTP083 ELISA Kit, MLCK ELISA Kit, smMLCK ELISA Kit, MYLK1 ELISA Kit, MLCK1 ELISA Kit, Telokin ELISA Kit, Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form ELISA Kit, Kinase-related protein ELISA Kit

Quick Database Links

UniProt

Q15746