Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L)

SKU: orb2302687

Description

This Multi-rAb antibody targets to Rabbit IgG (H+L) in ELISA and Western blot. It is higly specific to rabbit IgG and shows minimal reactivity for mouse, rat, human, pig, and bovine IgGs.

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution range: 1:10,000-1:100,000 for ELISA, 1:10,000-1:200,000 for western blotting with ECL substrates (1:10,000-1:40,000 is suggested for most system).
• Reactivity: Rabbit

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Goat
• Clonality: Recombinant
• Purity: The antibody was purified from culture media supernatant by immunoaffinity chromatography using Protein G beads.
• Conjugation: HRP

Storage & Handling

• Storage: Store at -20°C. Stable for one year after shipment.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 50% glycerol, 10 mg/mL rAlbumin, 0.1% Proclin-300, pH 7.4.
• Concentration: 1.0 mg/mL
• Disclaimer: For research use only

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TOM70 polyclonal antibody at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-PSMB1 polyclonal antibody at dilution of 1:10000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Beta Catenin polyclonal antibody at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-TDP43 recombinant antibody at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used at 1:20000 for detection.

PC-3 and Calyculin A treated PC-3 cell lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-Phospho-EIF4B (Ser406) Recombinant antibody at dilution of 1:20000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687), leading competitor’s non cross-adsorbed and cross-adsorbed secondary antibodies were all used at 0.05 μg/mL for detection.

HEK-293 and HepG2 cell lysates were subjected to SDS-PAGE followed by western blot with rabbit anti-ZO-1 polyclonal antibody at dilution of 1:50000. Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687), leading competitor’s non cross-adsorbed and cross-adsorbed secondary antibodies were all used at 0.05 μg/mL for detection.

Various lysates were subjected to SDS-PAGE followed by western blot with Beta tubulin rabbit recombinant antibody at dilution of 1:20000. Two batches of Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) were used at 1:20000 for detection.

Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (orb2302687) was used for detection. The result indicates that orb2302694 is highly specific for rabbit IgG and does not react with other species tested in the experiment.