MRC1 Rabbit Polyclonal Antibody

SKU: orb500928

Description

MRC1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human MRC1/CD206 (201-300/1456aa)
• Target: MRC1
• Molecular Weight: 180-200 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Mannose Receptor; C-type lectin domain family 13 member D; CD 206; CD206; CD206 antigen; CLEC13D; CLEC13DL; Macrophage mannose receptor; Mannose receptor C type 1; MMR; MRC 1; MRC-1; MRC1_HUMAN; Macrophage mannose receptor 1; MRC1L1; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1.

Blank control (blue line): Hep G2 (fixed with 70% ethanol Overnight at 4°C). Primary Antibody (green line): Rabbit Anti-Macrophage mannose receptor 1 antibody (orb500928), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-Macrophage mannose receptor 1 antibody (orb500928), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

MRC1

UniProt

P22897

References

Hao Xu 1 2, Xin Li 1 2, Wenxue Wang 1 2, Li Zhen 1 2, Baodong Zhao Strontium-Doped Marine Collagen Membranes Promote Osteogenesis by Inducing M2 Macrophage Polarization Tissue Eng Regen Med, (2025)

PubMed: 40658323