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| Catalog Number | orb257917 |
|---|---|
| Category | Proteins |
| Description | Mouse VEGF120 Protein, Tag Free is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 146 (Accession # AAB22254.1). |
| Target | VEGF120 |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Mouse |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | AAB22254.1 |
| MW | 14.1 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Expression Region | Ala 27 - Arg 146 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Cytokines |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

SDS-PAGE analysis of Mouse VEGF protein

Line graph illustrates about the sensitivity range of Mouse VEGF protein using different concentrations

Immobilized Mouse VEGF120, Tag Free (Cat. No. orb257917) at 2 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. orb1184768) with a linear range of 8-31 ng/mL (QC tested).
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
13.1 kDa | |
E.Coli |
>95%, determined by SDS-PAGE | |
Active Recombinant Mouse VEGF120 (ActiveMouse VEGF120) Ala 27 - Arg 146 (Accession # AAB22254.1 ) was expressed from HEK293 cells. |
Greater than 90.0% as determined by SDS-PAGE. | |
Escherichia Coli |
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