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Catalog Number | orb257917 |
---|---|
Category | Proteins |
Description | Mouse VEGF120 Protein, Tag Free is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 146 (Accession # AAB22254.1 ). |
Target | VEGF120 |
Conjugation | Unconjugated |
Tag | Native |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAB22254.1 |
UniProt ID | Q00731 |
MW | 14.1 kDa |
Application notes | This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse VEGF120, Tag Free (Cat. No. ) at 2 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. VE1-H52H9) with a linear range of 8-31 ng/mL (QC tested). |
Expression Region | Ala 27 - Arg 146 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAB22254.1 |
SDS-PAGE analysis of Mouse VEGF protein
Line graph illustrates about the sensitivity range of Mouse VEGF protein using different concentrations
Immobilized Mouse VEGF120, Tag Free (Cat. No. orb257917) at 2 μg/mL (100 μL/well) can bind Human VEGF R1 Protein, His Tag (Cat. No. orb1184768) with a linear range of 8-31 ng/mL (QC tested).
> 95%, determined by SDS-PAGE | |
Active Recombinant Mouse VEGF120 (ActiveMouse VEGF120) Ala 27 - Arg 146 (Accession # AAB22254.1 ) was expressed from HEK293 cells. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
13.1 kDa | |
E.Coli |
Greater than 90.0% as determined by SDS-PAGE. | |
Escherichia Coli |