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| Catalog Number | orb594838 |
|---|---|
| Category | Proteins |
| Description | Recombinant Mouse Interleukin-10(Il10) (Active) |
| Tag | Tag-Free |
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4 |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS |
| Protein Length | Full Length of Mature Protein |
| UniProt ID | P18893 |
| MW | 18.9 kDa |
| Application notes | Full Length of Mature Protein |
| Endotoxins | Less than 1.0 EU/µg as determined by LAL method. |
| Source | E.coli |
| Biological Origin | Mus musculus (Mouse) |
| Biological Activity | The ED50 as determined in a cell proliferation assay using FDC-P1 Mouse bone marrow cells is 6 ng/ml. |
| Expression Region | 19-178aa |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
| Alternative names | Interleukin-10;Il10;IL-10;Cytokine synthesis inhib Read more... |
| Research Area | Immunology & Inflammation |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
> 97% as determined by SDS-PAGE and HPLC. | |
18.7 kDa | |
E.Coli |
>90% as determined by SDS-PAGE. | |
21.05 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.5 kDa | |
E.Coli |
> 95% by SDS-PAGE. | |
Recombinant Mouse IL-10 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser19-Ser178) of mouse IL-10 (Accession #P18893) fused with an initial Met at the N-terminus. |
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