Mouse IgG2b Isotype Control Purified Antibody

• Catalog Number: orb154399
• Category: Antibodies
• Description: Mouse antibody to IgG2b which belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: Unconjugated
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: KLH-coupled trinitrophenol
• Tested applications: ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Application notes: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
• Antibody Type: Secondary Antibody
• Clone Number: MPC-11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG2b Isotype control (MPC-11) purified antibody (concentration in sample 2 μg/ml).