Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed

• Catalog Number: orb347433
• Category: Antibodies
• Description: MOUSE IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.
• Immunogen: Mouse IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000-1:100,000, IHC: 1:500-1:5,000, WB: 1:5,000-1:40,000
• Application notes: Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry western blotting as well as other HRP antibody based assays.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat Anti-Mouse IgG Secondary Antibody Peroxidase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA results of purified Goat anti-Mouse IgG Antibody Peroxidase Conjugated (Min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n orb346219). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin and TME ELISA Peroxidase Substrate (p/n orb348651).