Mouse IgG Fc Antibody Fluorescein Conjugated

• Catalog Number: orb347423
• Category: Antibodies
• Description: Mouse IgG F(c) antibody (FITC)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
• Immunogen: Mouse IgG F(c) fragment
• Tested applications: DOT, FC, FLISA, IF, WB
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Application notes: Anti-Mouse IgG F(c) Fluorescein conjugated Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Goat Anti-Mouse IgG F(c) peroxidase Conjugated Ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot of Fluorescein conjugated Goat Anti-Mouse IgG F(c) secondary antibody. Lane 1: Mouse IgG F(c). Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Mouse IgG F(c). Other band(s): None.