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Catalog Number | orb1926698 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB51264 |
Tested applications | FC, WB |
Predicted Reactivity | Rat |
Reactivity | Mouse |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, FC: 1:25 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 35019 Da |
Target | This Mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam. |
UniProt ID | Q99JW5 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Epithelial cell adhesion molecule, Ep-CAM, Epithel Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of HepG2 cells using (Mouse) Epcam Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from mouse kidney, mouse lung, mouse testis tissue lysate (from left to right), using Epcam Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
FC, IHC-P, WB | |
Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |