MEK3 (MAP2K3) Antibody (N-term)

SKU: orb1928768

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This MEK3 (MAP2K3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human MEK3 (MAP2K3). Antigen Region: 15-45 aa.
• Target: MAP2K3
• Molecular Weight: 39318 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, MEK 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK-2, SAPKK2, MAP2K3, MEK3, MKK3, PRKMK3, SKK2

Western blot analysis of anti-MKK3 Pab in Jurkat cell lysate. MKK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

IHC of matched glioma core (top row) and rim (middle row) samples as well as normal brain control (bottom row) from a glioma invasion specific tissue microarray. Insets, individual tumor cells (black squares) are presented at higher magnification to facilitate interpretation of the staining pattern. Staining for MKK3 and pMKK3 exhibits strong signal in majority of invasive cells (B) compared with stationary cells from tumor core (A). Normal brain control shows only weak staining in reactive astrocytes (C).

Quick Database Links

Gene Symbol

MAP2K3

UniProt

P46734

RefSeq (Protein):NP_002747.2, NP_659731.1