MDA5 Antibody

• Catalog Number: orb1238796
• Category: Antibodies
• Description: MDA5 Antibody
• Target: IFIH1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: MDA5 Antibody is affinity chromatography purified via peptide column.
• Immunogen: MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5.The immunogen is located within the last 50 amino acids of MDA5.
• UniProt ID: Q9BYX4
• MW: Predicted: 113 kDa Observed: 115 kDa
• Tested applications: ELISA, IF, IHC-P, WB
• Application notes: MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MDA5 Antibody: Hlcd, MDA5, MDA-5, RLR-2, IDDM19, R
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: NP_071451
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 µg/mL.

Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 µg/ml.

Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.

Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 µg/mL.