LRP1 Rabbit Polyclonal Antibody

• Catalog Number: orb6328
• Category: Antibodies
• Description: LRP1 Rabbit Polyclonal Antibody
• Target: LRP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human LRP1 (4451-4545/4545aa)
• UniProt ID: Q07954
• RRID: AB_10940409
• MW: 85/515 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: A2MR; alpha 2 macroglobulin receptor; alpha 2MR; A
• Background: Endocytic receptor involved in endocytosis and in
• Research Area: Alzheimer's Disease, Apoptosis, Cancer Research, C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min). Primary Antibody: Rabbit Anti-LRP1 antibody (orb6328), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: U2os (Human) Cell Lysate at 30 ug, Primary: Anti-LRP1 (orb6328) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.

Tissue/Cell: Mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LRP1 Polyclonal Antibody, Unconjugated (orb6328) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Mouse lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-LRP1 Polyclonal Antibody, Unconjugated (orb6328) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.