LAMP2 Antibody (Center)

SKU: orb1933328

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD35498
• Immunogen: This LAMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the Central region of human LAMP2. Antigen Region: 196-225 aa.
• Target: LAMP2
• Molecular Weight: 44961 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, CD107b, LAMP2

Western blot analysis of LAMP2 (arrow) using LAMP2 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LAMP2 gene (Lane 2). Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides with a total molecular weight of ~100-110 kDa.

Anti-LAMP2 Antibody (Center) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

LAMP2

UniProt

P13473

RefSeq (Protein):NP_002285.1, NP_001116078.1, NP_054701.1