Lamin A / C Antibody

• Catalog Number: orb1258730
• Category: Antibodies
• Description: Lamin A / C Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide of human Lamin A / C
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 74kDa
• Target: LMNA
• UniProt ID: P02545
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC,
• Note: For research use only
• Application notes: WB: 1:200 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (orb1258730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded human mammary cancer using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Lamin A/C antibody (orb1258730) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody (orb1254718) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using Lamin A/C antibody (orb1258730) at dilution of 1:100. Blue: DAPI for nuclear staining.