Lactic acid

SKU: orb1680007

Description

Lactic acid (2-hydroxypropanoic acid) is an endogenous agonist of the hydroxycarboxylic acid receptor 1 (HCAR1) with an EC50 of 4.2-5 mM. This glycolysis product is utilized in research areas including immunometabolism and tumor biology, serving as a key metabolite for in vitro and in vivo studies of cellular energetics and signaling.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 50-21-5
• MW: 90.08
• Purity: 99.45% (May vary between batches)
• Formula: C₃H₆O₃
• SMILES: CC(O)C(O)=O
• Target: Others,HCAR,Endogenous Metabolite
• Solubility: DMSO:90 mg/mL (999.11 mM);H2O:100 mg/mL (1110.12 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:5 mg/mL (55.51 mM)

Bioactivity

• Target IC50:
  GPR109B:1 mM (EC50)|GPR109A:1 mM (EC50)|GPR81 (HCAR1):1 mM (EC50)
• In Vivo:
  METHODS: To detect the anti-tumor activity in vivo, Lactic acid (1.6 g/kg) was subcutaneously injected into Female C57BL/6 mice with MC38 colon cancer cells; pH 7.4 Once a day; From Day 8 to Day 30. RESULTS: Lactic acid demonstrated CD8+ T cell-dependent tumor growth inhibition in the MC38 colon cancer model.
• In Vitro:
  METHODS: : Effects on cell proliferation and migration of uveal melanoma after treatment with Lactic acid (20 mM) for 24 and 48 hours. RESULTS: Lactic acid led to a significant reduction in cell proliferation and migration. Method: Uveal melanoma was treated with Lactic acid (20 mM) for 24 hours, and the protein expression level was detected by western blot. Result: Lactic acid promoted the increase in the protein expression levels of MCT1 and HCAR1.

Storage & Handling

• Storage: Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2-hydroxypropanoic acid, Lactic acid

Compound Identifiers

PubChem CID

612