KRT20/CK20/Cytokeratin 20 Antibody

SKU: orb1537291

Description

KRT20/CK20/Cytokeratin 20 Antibody

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: ELISA, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 49kD band observed in Human Colon and Ileum lysates (calculated MW of 48.5kD according to NP_061883.1). Recommended concentration: 0.01-0.03 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-ETNAPRAGRDYS, from the internal region of the protein sequence according to NP_061883.1.
• Target: KRT20 / CK20 / Cytokeratin 20
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KRT20, Cytokeratin-20, Cytokeratin 20, CK20, K20, Keratin 20, Keratin-20, KRT21, CK-20, Protein IT

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT20 antibody (0.03 ug/ml) staining of Human Colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

KRT20 / CK20 / Cytokeratin 20