[KO Validated] SOD2 Rabbit pAb Discontinued

SKU: orb48543

Description

Rabbit polyclonal antibody to SOD2

Research Area

Cardiovascular Disease, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:10-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
• Molecular Weight: 25kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.05% proclin300,50% glycerol,pH7.3.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SOD2 antibody, anti IPOB antibody, anti MNSOD antibody, anti MVCD6 antibody

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SOD2 Rabbit pAb(orb48543) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using [KO Validated] SOD2 Rabbit pAb(orb48543) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded rat ovary using SOD2 Rabbit pAb (orb48543) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot analysis of lysates from wild type(WT) and SOD2 knockout (KO) 293T cells, using SOD2 Rabbit pAb (orb48543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of various lysates using [KO Validated] SOD2 Rabbit pAb (orb48543) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Quick Database Links

UniProt

P04179

Zhang, Yinliang et al. Celastrol ameliorates liver metabolic damage caused by a high-fat diet through Sirt1 Mol Metab, 6, 138-147 (2017)

PubMed: 28123944