Keratin-pan rabbit pAb

• Catalog Number: orb771505
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Keratin-pan
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Synthetic peptide from human protein at AA range: 150-220
• Concentration: 1 mg/ml
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Conjugation: Unconjugated
• MW: 70
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse-lung mouse-brain mouse-kidney mouse-heart lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).