Keratin-pan rabbit pAb

SKU: orb771505

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Description

Rabbit polyclonal antibody to Keratin-pan

Images & Validation

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Tested Applications	ELISA, WB
Dilution range	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	Synthetic peptide from human protein at AA range: 150-220
Molecular Weight	70
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

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Western blot analysis of mouse-lung mouse-brain mouse-kidney mouse-heart lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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