IL11 Antibody (N-Term)

SKU: orb1925503

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB56890
• Immunogen: This IL11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 27-61 amino acids from human IL11.
• Target: IL11 (HGNC:5966)
• Molecular Weight: 21429 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, Oprelvekin, IL11

Anti-IL11 Antibody (N-Term) at 1:2000 dilution + DU145 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing PC-3 cells stained (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

IL11 (HGNC:5966)

UniProt

P20809