IL-33 Antibody [IL33305B]

SKU: orb1272817

Description

IL-33 Antibody [IL33305B]

Images & Validation

• Tested Applications: ELISA, FA, IHC, IP, WB
• Reactivity: Human
• Application Notes:
  ELISA: (direct or indirect: 1:2'000-1:5'000), Functional Application (Blocking), Immunohistochemistry (paraffin sections (1:50-1:200)), Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2aκ
• Clone No.: IL33305B
• Immunogen: Recombinant human IL-33.
• Purification: Protein G-affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Interleukin-33; IL-1F11; NF-HEV

Immunohistochemical staining of human tissue using anti-IL-33 (human), mAb (IL33305B) (Prod. No. orb1272817) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour). B. Isotype control with IgG2a (negative control)

Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using anti-IL-33 (human), mAb (IL33305B) (Prod. No. orb1272817). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml) ; 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to thewells followed by washing; 3) add anti-FLAG HRP conjugated (1:2000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm

Immunoprecipitation of recombinant hIL-33 proteins using anti-IL-33 (human), mAb (IL33305B) (orb1272817). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.

Western blot analysis of human IL-33 using anti-IL-33 (human), mAb (IL33305B) (orb1272817) at 1:2000 dilution. 1. Recombinant human IL-33 (His tagged). 2. Recombinant human IL-33 (FLAG?-tagged). 3. Recombinant mouse IL-33 (FLAG?-tagged). 4. Other protein (His tagged, negative control).