IFNAR2 Rabbit Polyclonal Antibody

• Catalog Number: orb100572
• Category: Antibodies
• Description: IFNAR2 Rabbit Polyclonal Antibody
• Target: IFNAR2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from mouse IFNAR2 (165-260/513aa)
• MW: 54 kDa
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, ELISA=1:1000-5000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFN alpha REC; IFN-alpha binding protein; IFN-alph
• Research Area: Immunology, JAK/ STAT Signaling Pathway, SARS /COV
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hayashi, Yushi et al. Cochlear supporting cells function as macrophage-like cells and protect audiosensory receptor hair cells from pathogens Sci Rep, 10, 6740 (2020)

PubMed: 32317718

Applications

IHC

Reactivity

Mouse

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-IFNAR2/IFNABR Polyclonal Antibody, Unconjugated (orb100572) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.