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| Catalog Number | orb257918 |
|---|---|
| Category | Proteins |
| Description | Human VEGF121 Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 147 (Accession # P15692-9). |
| Target | VEGF121 |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | P15692 |
| MW | 14.1 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 14.1 kDa. The protein migrates as 15-16 kDa and 17-20 kDa under reducing (R) condition, and 30-40 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Expression Region | Ala 27 - Arg 147 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Cytokines |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Stafford, Ryan L. et al. In vitro Fab display: a cell-free system for IgG discovery Protein Eng Des Sel, 27, 97-109 (2014)

SDS-PAGE analysis of Human VEGF protein

Line graph illustrates about the sensitivity range of Human VEGF protein using different concentrations

Immobilized Human VEGF121 Protein, premium grade (Cat. No. orb257918) at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag with a linear range of 0.15-2.5 μg/mL (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 40.2 kDa after removal of the signal peptide. The apparent molecular mass of VEGF121-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
>92%, determined by SDS-PAGE | |
This protein contains the human VEGF 121 isoform (P15692-9) (Met 1-Arg 147) was expressed from Baculovirus-Insect Cells. |
>95%, determined by SDS-PAGE | |
This protein contains the human VEGF 121 isoform (P15692-9) (Met 1-Arg 147) was expressed from Baculovirus-Insect Cells. |
>(55.6 + 41.6)%, determined by SDS-PAGE | |
This protein contains the human VEGF 121 isoform (P15692-9) (Met 1-Arg 147) was expressed from Baculovirus-Insect Cells. |
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