Human VEGF R3 Protein, Fc Tag

• Catalog Number: orb18510
• Category: Proteins
• Description: Human VEGF R3, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # NP_002011.2).
• Target: VEGF R3
• Conjugation: Unconjugated
• Tag: C-Fc
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH 7.5
• Purity: 95%
• UniProt ID: NP_002011.2
• RRID: AB_10746635
• MW: 111.2 kDa
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 111.2 kDa. The protein migrates as 140-160 kDa under reducing (R) condition, and 280-320 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Expression Region: Tyr 25 - Ile 776
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Disease Biomarkers
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human VEGF R3 protein

Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).