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Human VEGF-D Protein, His Tag
Catalog Number: orb257928
| Catalog Number | orb257928 |
|---|---|
| Category | Proteins |
| Description | Human VEGF-D, His Tag is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # AAH27948). |
| Target | VEGF-D |
| Conjugation | Unconjugated |
| Tag | C-9×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| Protein Sequence | AAH27948 |
| UniProt ID | O43915 |
| MW | 13.4 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. ) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
| Expression Region | Phe 93 - Ser 201 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH27948 |
SDS-PAGE analysis of Human VEGF-D protein
Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Human VEGFD Protein, His Tag [orb2697434]
> 98%, determined by SDS-PAGE
This protein contains the mature form of human VEGFD (NP_0044601) corresponding to amino acid (Phe 93-Ser 201) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells.
50 μg, 20 μgHuman VEGFD Protein, His Tag [orb2701423]
> 95%, determined by SDS-PAGE and SEC-HPLC
This protein contains the mature form of human VEGFD (NP_0044601) corresponding to amino acid (Phe 93-Ser 201) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells.
100 μg, 50 μgRecombinant Human VEGF-D Protein, His Tag [orb1550853]
> 95% by SDS-PAGE.
Recombinant Human VEGF-D Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Phe93-Ser201) of human VEGF-D (Accession #O43915) fused with a 6��His Tag at the C-terminus.
50 μg
