Human TNF-alpha Protein, His Tag

SKU: orb383503

Description

Human TNF-alpha Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: TNF-alpha
• Reactivity: Human
• Tag: C-6×His
• Molecular Weight: 18.3 kDa
• Expression Region: Val 77 - Leu 233
• Purity: >95% as determined by SDS-PAGE and SEC-MALS.
• Endotoxins: Less than 1.0 EU per μg by the LAL method / rFC method.
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

SDS-PAGE analysis of Human TNF-alpha protein

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag (Cat. No. orb383503) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Quick Database Links

Gene Symbol

TNF-alpha

UniProt

NP_000585.2

RefSeq (Protein):NP_000585.2