Human TGF-beta 1 Protein

• Catalog Number: orb257996
• Category: Proteins
• Description: Human TGFB1, premium grade is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390.
• Target: TGF-beta 1
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN with trehalose as protectant
• Purity: 95%
• UniProt ID: P01137
• MW: 12.8 kDa (monomer)
• Application notes: This protein carries no "tag". The protein has a calculated MW of 12.8 kDa (monomer). The protein migrates as 14 kDa (monomer) under reducing (R) condition, and 25 kDa (Dimer) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE)
• Expression System: HEK293
• Endotoxins: Less than 0.1 EU per μg by the LAL method / rFC method
• Expression Region: Ala 279 - Ser 390
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

References

Cirka, Heather et al. Active Traction Force Response to Long-Term Cyclic Stretch Is Dependent on Cell Pre-stress Biophys J, 110, 1845-1857 (2016)

PubMed: 27119644

Immobilized Human TGFB1, premium grade (Cat. No. orb257996) at 0.2 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag (Cat. No. orb257866) with a linear range of 0.2-2.5 ng/mL (QC tested).

Human TGF-beta 1 Protein (Cat. No. orb257996) immobilized on CM5 Chip can bind Human TGF-beta RI Protein, Fc Tag (Cat. No. orb1786177) with an affinity constant of 99.2 nM as determined in a SPR assay (Biacore 8K)

Human TGF-beta 1 Protein (Cat. No. orb257996) inhibits the Human IL-4, premium grade (Cat. No. orb257598) dependent proliferation the TF-1 cells. The specific activity of Human TGF-Beta 1 Protein, Tag Free is > 8.00ⅹ10^6 IU/mg, which is calibrated against transforming growth factor β1 (NIBSC code: 89/514)

Human TGF-beta 1 Protein (Cat. No. orb257996) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).