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Catalog Number | orb1496186 |
---|---|
Category | Proteins |
Description | Oncostatin-M-specific receptor subunit beta(OSMRB) alos know as Interleukin-31 receptor subunit beta (IL-31RB), is an alternative subunit (OSMRβ) for an OSM receptor complex (a heterodimer of gp130 and OSMRβ), that is activated by OSM but not by LIF. OSMR beta associates with the low affinity OSM·gp130 complex to form a high affinity heterodimeric receptor that is capable of transducing OSM-specific signaling events. |
Target | OSMR |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | Glu 28 - Met 740 |
UniProt ID | Q99650 |
MW | 83.0 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.0 kDa. The protein migrates as 105-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Glu 28 - Met 740 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 81.9 kDa after removal of the signal peptide. The apparent molecular mass of OSMR-His is approximately 100-250 kDa due to glycosylation. | |
Mammalian |
> 96%, determined by SDS-PAGE | |
This protein contains the human OSMR (NP_0039901) extracellular domain (Met 1-Met 740) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
≥90% as determined by SDS-PAGE | |
This protein contains the human OSMRB(Glu28-Ser739) was fused with the C-terminal His Tag and expressed in E. coli. |
> 92% by SDS-PAGE. | |
Recombinant Human OSMR Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu28-Ser739) of human OSMR (Accession #NP_003990) fused with a 6��His Tag at the C-terminus. |