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Human NKp46 Protein, Fc Tag

Catalog Number: orb257704

DispatchUsually dispatched within 5-10 working days
$ 590.00
Catalog Numberorb257704
CategoryProteins
DescriptionHuman NKp46, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asn 254 (Accession # AAH64806).
TargetNKp46
ConjugationUnconjugated
TagC-Fc
ReactivityHuman
Form/AppearancePowder
Buffer/Preservatives50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH 7.5
Purity95%
Protein SequenceAAH64806
UniProt IDO76036
MW53.0 kDa
Application notesThis protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression SystemHEK293
Endotoxins0.01 EU per μg
Biological OriginHuman
Biological ActivityImmobilized Human NKp46, Fc Tag, premium grade (Cat. No. ) at 1 μg/mL (100 μL/well) can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Expression RegionGln 22 - Asn 254
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
NoteFor research use only
NCBIAAH64806
Human NKp46 Protein, Fc Tag

SDS-PAGE analysis of Human NKp46 protein

Human NKp46 Protein, Fc Tag

Immobilized Human NKp46, Fc Tag, premium grade (Cat. No. orb257704) at 1 μg/mL (100 μL/well) can bind Anti-NKP46 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).

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    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 52.5 kDa after removal of the signal peptide. The apparent molecular mass of NCR1-hFc is approximately 55-70 kDa due to glycosylation.

    Mammalian

    10 μg, 100 μg, 50 μg
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    ELISA,  MS,  SDS-PAGE,  WB

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    Human cells

    50 μg, 500 μg, 10 μg
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    500 μg, 1 mg, 50 μg, 10 μg
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    Unconjugated

    90%

    53.0 kDa

    1 mg, 100 μg
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    Unconjugated

    90%

    36.4 kDa

    1 mg, 100 μg