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| Catalog Number | orb257990 |
|---|---|
| Category | Proteins |
| Description | Human LIF Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # AAH69540.1). |
| Target | LIF |
| Conjugation | Unconjugated |
| Tag | Native |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| UniProt ID | AAH69540.1 |
| MW | 19.9 kDa |
| Application notes | This protein carries no "tag". The protein has a calculated MW of 19.9 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.1 EU per μg |
| Expression Region | Ser 23 - Phe 202 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Cytokines |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

SDS-PAGE analysis of Human LIF protein

Immobilized Human LIF Protein, premium grade (Cat. No. orb257990) at 0.2 μg/mL (100 μL/well) can bind Human LIF R, Fc Tag (Cat. No. orb257674) with a linear range of 4-63 ng/mL (QC tested).
Human | |
0.32-20ng/mL | |
0.19 ng/mL |
> 98% as determined by SDS-PAGE and HPLC. | |
19.7 kDa | |
E.Coli |
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