Recombinant Human IL1B Protein, hFc Tag

• Catalog Number: orb1173751
• Category: Proteins
• Description: Recombinant Human IL1B Protein with C-terminal human Fc tag
• Target: IL1B
• Tag: C-Human Fc Tag
• Reactivity: Human
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P01584
• MW: The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation.
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: IL-1;IL1-BETA;IL1beta;IL1F2
• Research Area: Immunology
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Recombinant Human IL1B Protein, hFc Tag on SDS-PAGE under reducing condition

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Recombinant Human IL1B Protein, hFc Tag (orb1173751) can bind IL1B Biosimilar Antibody(orb1291211) in a linear range of 0.61–156.25 ng/mL

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL1R2 Protein, His Tag (orb1290929) can bind Recombinant Human IL1B Protein, hFc Tag (orb1173751) in a linear range of 3.20–400 ng/mL

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Recombinant Human IL1B Protein, hFc Tag (orb1173751) can bind Human IL1RA Protein, mFc Tag (orb2321379) in a linear range of 0.64–80 ng/mL