Human IL-6 Protein

• Catalog Number: orb257601
• Category: Proteins
• Description: Human IL-6, premium grade is expressed from human 293 cells (HEK293). It contains AA Val 30 - Met 212 (Accession # NP_000591.1).
• Target: IL-6
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• UniProt ID: NP_000591.1
• MW: 20.8 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.01 EU per μg
• Expression Region: Val 30 - Met 212
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Cytokines
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL6 protein

Human IL-6, stimulates proliferation of TF-1 human erythroleukemic cell line. The specific activity of Human IL-6 is > 1.00×10^8 IU/mg, which is calibrated against human IL-6 WHO International Standard

The Cell based assay shows that Human IL-6 Protein is stable at 37°C for 48 hours